object BasecallingParams extends Serializable

Contains methods useful for extractin basecalling paramter information.

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  5. def bamFileFrom(output: FgBioDef.DirPath, sample: Sample, lane: Int): FgBioDef.PathToBam

    Produces the path to the BAM file for the given sample and lane.

    Produces the path to the BAM file for the given sample and lane.

    The BAM file name will <sample-name>.<barcode-seq>.<lane>.bam. The resulting file name will be sanitized to remove problematic characters.

    output

    the base output directory.

    sample

    the sample.

    lane

    the lane number.

    returns

    the path to the BAM file for the given sample and lane.

  6. def barcodeFileFrom(output: FgBioDef.DirPath, lane: Int): FgBioDef.FilePath

    The lane-specific file containing information necessary to run Picard's ExtractIlluminaBarcodes and CollectIlluminaBasecallingMetrics.

    The lane-specific file containing information necessary to run Picard's ExtractIlluminaBarcodes and CollectIlluminaBasecallingMetrics.

    output

    the base output directory.

    lane

    the lane number.

    returns

    the path to the barcode file, named "barcode_data.<lane>.txt".

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  11. def from(sampleSheet: SampleSheet, lanes: Seq[Int], output: FgBioDef.DirPath, bamOutput: Option[FgBioDef.DirPath] = None): Seq[BasecallingParams]

    Extracts sample and library information from an sample sheet for a given lane.

    Extracts sample and library information from an sample sheet for a given lane. The resulting files are used to run Picard's ExtractIlluminaBarcodes, CollectIlluminaBasecallingMetrics, and IlluminaBasecallsToSam. See ExtractBasecallingParamsForPicard for more information.

    sampleSheet

    the Illumina Experiment Manager sample sheet.

    lanes

    the lane number(s) (1-based).

    output

    the output directory where the barcode, library param, and BAM files should be written.

    bamOutput

    the optional output directory to where per-lane BAM files will be written, otherwise use output.

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  15. def libraryParamsFileFrom(output: FgBioDef.DirPath, lane: Int): FgBioDef.FilePath

    The lane-specific file containing information necessary to run Picard's IlluminaBasecallsToSam.

    The lane-specific file containing information necessary to run Picard's IlluminaBasecallsToSam.

    output

    the base output directory.

    lane

    the lane number.

    returns

    the path to the library params file, named "library_params.<lane>.txt".

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